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crop seq car puro vector  (Addgene inc)


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    Structured Review

    Addgene inc crop seq car puro vector
    Crop Seq Car Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crop+seq+car+puro+vector/pGSTag+BimL+(T56A)+(Plasmid+%2324253)/pm40993398-475-19-21
    Average 93 stars, based on 2 article reviews
    crop seq car puro vector - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

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    Article Title: Systematic discovery of CRISPR-boosted CAR T cell immunotherapies.
    Article Snippet: This cloning yielded the pPD0010_CROP-seq-CAR-mCherry2 vector (Addgene, 242533), from which all CROP-seq-CAR variants were derived. .. For efficient selection of transduced cells, mCherry was replaced with the PAC antibiotic resistance gene, resulting in the pPD0039_ CROP-seq-CAR-Puro vector (Addgene, 242532) used in most screens. .. To generate variants with different CARs, this vector was linearized by PCR using Q5 High-Fidelity 2X Master Mix (NEB, M0492L) and primers prCART0026 and prCART0028, which exclude the sequence for the CD19 scFv.

    Plasmid Preparation:

    Article Title: Systematic discovery of CRISPR-boosted CAR T cell immunotherapies.
    Article Snippet: This cloning yielded the pPD0010_CROP-seq-CAR-mCherry2 vector (Addgene, 242533), from which all CROP-seq-CAR variants were derived. .. For efficient selection of transduced cells, mCherry was replaced with the PAC antibiotic resistance gene, resulting in the pPD0039_ CROP-seq-CAR-Puro vector (Addgene, 242532) used in most screens. .. To generate variants with different CARs, this vector was linearized by PCR using Q5 High-Fidelity 2X Master Mix (NEB, M0492L) and primers prCART0026 and prCART0028, which exclude the sequence for the CD19 scFv.

    other:

    Article Title: Systematic discovery of CRISPR-boosted CAR T cell immunotherapies.
    Article Snippet: To generate variants with different CARs, this vector was linearized by PCR using Q5 High-Fidelity 2X Master Mix (NEB, M0492L) and primers prCART0026 and prCART0028, which exclude the sequence for the CD19 scFv.



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